Coding

Part:BBa_K339001:Design

Designed by: Emily Hicks   Group: iGEM10_Calgary   (2010-10-23)

Mutant Maltose Binding Protein (malE31)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 435
    Illegal BamHI site found at 171
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 133


Design Notes

Source

Plasmid DNA was obtained from Jean-Betton labs in France, amplified out of the plasmid through PCR and then subsequently biobricked (Betton et al., 2002).

References

Betton, J., Phichith, D. and Hunke, S. (2002). Folding and aggregation of export-defective mutants of the maltose-binding protein. Research in Microbiology. (153): 399-404.

Kapust, R. and Waugh, D. (1999). Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Science. 8(8):1688-1674.

ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Maryland 21702-1201, USA.